Effects of fish farm activities on sessile benthic taxa and their associated microbiota: A case study with Weberella bursa

Project Title

Effects of fish farm activities on sessile benthic taxa and their associated microbiota: A case study with Weberella bursa

Organism

marine metagenome

Organization

Institute of Marine Research

SRA Runs

22

Samples

12

Data

454,698,027 reads, 137.71 Gbp

Description

In this study, we aimed to begin to address the knowledge gap on how sessile benthic organisms living on mixed or hard-substrate habitats respond to the effects of salmon farming. To do so, Weberella bursa specimens, which are among the most common sponges in the Nordic Seas (Plotkin et al., 2018), were translocated along a distance gradient from a salmon farm located in the Sognefjord region along the mid-west coast of Norway. After being exposed for a period of seven months, these specimens were retrieved and their tissue analysed via 1) transcriptomics, for gene expression analysis, 2) 16S rRNA metabarcoding, for characterizing associated taxonomic and functional microbial communities, 3) gas chromatography, for fatty acid profiling, and 4) ratio mass spectrometer for carbon and nitrogen stable isotope analysis. Specifically, our objectives were to find out 1) how these layers of information correlate to each other, 2) how sensitive they are to salmon farming, and 3) whether we could identify meaningful potential bio-indicators of fish farm activities that could be subsequently used in routine monitoring programs.

bacterial 16S rRNA · bacterial 16S 787-357

Forward

GGACTACNVGGGTWTCTAAT

Reverse

ACTCCTACGGGAGGCAGCAG

Samples

10 · primers in 99.9% of reads, median across the assay's samples

449 ASVs across the run.

Container image

illumina-amplicon

9ffda89262b7

Cluster

grex