Microbial stowaways: Exploring shipwreck microbiomes in the deep Gulf of Mexico

PRJNA599410 · illumina_amplicon · Created 2026-08-18

Ready
✓ Data ✓ Pipeline
1

Data Selected

Locked

Your BioProject data has been linked and a pipeline selected.

Project metadata
Project Title
Microbial stowaways: Exploring shipwreck microbiomes in the deep Gulf of Mexico
Organism
marine metagenome, marine sediment metagenome
Organization
University of Southern Mississippi
SRA Runs
170
Samples
170
Data
12,227,694 reads, 7.3 Gbp
Description
DNA collected from sediments and water column around two shipwrecks in the Gulf of Mexico as a part of "Microbial stowaways: Exploring shipwreck microbiomes in the deep Gulf of Mexico" project supported by NOAA Ocean Exploration and Research. This study investigates the impact of historic shipwrecks on microbial community and structure on the seafloor.
Selected data types
PlatformILLUMINA
StrategyAMPLICON
SourceGENOMIC
Runs170
Data7.3 Gbp
2

Pipeline Analysis

Done

Processed with illumina_amplicon on Alliance Canada HPC.

Explore Data on microscape.app → Download for offline use
Assays and primers used

inferred primers

Forward
AATYGGANTCAACGCCRGAAAWCTYACC
Reverse
TGWGTRCAAGRCYCRRGAACRYATTCAC
Samples
81 · primers in 61.6% of reads, median across the assay's samples

inferred primers

Forward
ACGCGHNRAACCTTACC
Reverse
GTGWGTRCAAGRCCCGGGAACGTATTCA
Samples
74 · primers in 61.2% of reads, median across the assay's samples

inferred primers

Forward
ACGCGHNRAACCTTACC
Reverse
TGWGTRCAAGRCYCRRGAACRYATTCAC
Samples
11 · primers in 51.3% of reads, median across the assay's samples

inferred primers

Forward
AATYGGANTCAACGCCRGAAAWCTYACC
Reverse
GTGWGTRCAAGRCCCGGGAACGTATTCA
Samples
4 · primers in 59.2% of reads, median across the assay's samples

31,485 ASVs across the run.

Pipeline info

Container image

illumina-amplicon
05f23de10d82
Cluster
grex