Prokaryotic communities associated with different habitats within coral animals

PRJNA324417 · illumina_amplicon · Created 2026-08-18

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Project metadata
Project Title
Prokaryotic communities associated with different habitats within coral animals
Organism
coral metagenome, marine metagenome
Organization
Woods Hole Oceanographic Institution
SRA Runs
160
Samples
160
Data
24,799,382 reads, 12.4 Gbp
Description
The diverse prokaryotic communities associated with reef-building corals may provideimportant ecological advantages to their threatened hosts. The consistency ofrelationships between corals and specific prokaryotes, however, is debated and thelocations where microbially mediated processes occur in the host are not yet resolved.Here, we used physical and chemical separation of coral mucus and tissue followed bysequencing of partial small subunit ribosomal RNA genes of bacteria and archaea todifferentiate the mucus- and tissue-specific core symbionts of five common Caribbeancorals (Porites astreoides, P. porites, Orbicella faveolata, Montastrea cavernosa andDiploria strigosa).
Selected data types
PlatformILLUMINA
StrategyAMPLICON
SourceMETAGENOMIC
Runs160
Data12.4 Gbp
2

Pipeline Analysis

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Processed with illumina_amplicon on Alliance Canada HPC.

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Assays and primers used

inferred primers

Forward
CGGAGGRBKCRAGCGTTRKYCGGAWTTA
Reverse
CTCCCCACSCTYTCGTRYYT
Samples
52 · primers in 65.8% of reads, median across the assay's samples

inferred primers

Forward
TTRWYCGGAWTYAYTGGGCGTAAA
Reverse
CTCCCCACSCTYTCGTRYYT
Samples
41 · primers in 57.1% of reads, median across the assay's samples

inferred primers

Forward
WSGSAARRGCGTAAAG
Reverse
CTCCCCACSCTYTCGTRYYT
Samples
19 · primers in 79.7% of reads, median across the assay's samples

28,815 ASVs across the run.

Pipeline info

Container image

illumina-amplicon
05f23de10d82
Cluster
grex