Microbial stowaways: Exploring shipwreck microbiomes in the deep Gulf of Mexico

PRJNA599410 · illumina_amplicon · Created 2026-08-19

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Project metadata
Project Title
Microbial stowaways: Exploring shipwreck microbiomes in the deep Gulf of Mexico
Organism
marine metagenome, marine sediment metagenome
Organization
University of Southern Mississippi
SRA Runs
170
Samples
170
Data
12,227,694 reads, 7.3 Gbp
Description
DNA collected from sediments and water column around two shipwrecks in the Gulf of Mexico as a part of "Microbial stowaways: Exploring shipwreck microbiomes in the deep Gulf of Mexico" project supported by NOAA Ocean Exploration and Research. This study investigates the impact of historic shipwrecks on microbial community and structure on the seafloor.
Selected data types
PlatformILLUMINA
StrategyAMPLICON
SourceGENOMIC
Runs170
Data7.3 Gbp
2

Pipeline Analysis

Done

Processed with illumina_amplicon on Alliance Canada HPC.

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Assays and primers used

eukaryotic 18S rRNA · V6-V8 · eukaryotic 18S 1181-1638

Forward
TYAATYGGANTCAACGCSWKRAA
Reverse
CRGTGWGTRCAAGGVSCRGGRAC
Samples
170 · primers in 99.8% of reads, median across the assay's samples

16S rRNA · V6-V8 · bacterial 16S 969-1406 · Bacteria/Archaea

Forward
ACGCGHNRAACCTTACC
Reverse
CRGTGWGTRCAAGGVSCRGGRAC
Samples
168 · primers in 99.9% of reads, median across the assay's samples

26,702 ASVs across the run.

Pipeline info

Container image

illumina-amplicon
9ffda89262b7
Cluster
grex